Characterizing Stroke Clots Using Single-Cell Sequencing.

ABSTRACT:

Background

Ischemic stroke results in significant morbidity and mortality. By examining gene expression of cells comprising stroke clots, we aim to gain valuable insights into the underlying mechanisms of this disease and identify potential biomarkers of stroke cause.

Methods

We employed single-cell RNA sequencing to analyze 10 clot samples from patients diagnosed with large vessel occlusion stroke. We aimed to identify and compare the immune cell compositions and gene expression profiles between stroke clots (atrial fibrillation vs carotid atherosclerosis). We also used Multi-marker Analysis of Genomic Annotation and genome-wide association studies summary statistics from the GIGASTROKE consortium to assess associations between genetic variants and cell type-specific gene e

SUBMITTER: Renedo D 

PROVIDER: S-EPMC12553457 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature