Putative membrane protein Cgl2168 and its variant enhance thermotolerance in <i>Corynebacterium glutamicum</i>.

ABSTRACT: Corynebacterium glutamicum ATCC 13032 is widely used as the chassis strain for industrial production of various amino acids, but its thermotolerance limits productivity under high temperatures. Comparative genomic analysis in this study identified cgl2168 as a key determinant of enhanced thermotolerance in a laboratory strain (13032-JN) compared to another stock (13032-TJ). A guanine at Residue 270 (G270) deletion in cgl2168 (cgl2168 ^Δ270G) in 13032-JN significantly improved growth at 40 °C, while reverting this mutation or deleting cgl2168 impaired thermotolerance. Structural analysis revealed the deletion extends the C-terminus with an additional α-helix. Transcriptomic profiling showed cgl2168 ^Δ270G increased gene expressio

SUBMITTER: Gao N 

PROVIDER: S-EPMC12554045 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature