Tumor-derived exosomal miR-199b-5p promotes proliferation and epithelial-mesenchymal transition in non-small cell lung cancer by targeting CCNL1.

ABSTRACT:

Objective

To explore the effect of exosome-mediated miR-199b-5p on lung cancer cells behavior, intrapulmonary metastasis, and its underlying mechanism.

Methods

Exosomes from SK-LU-1 cells overexpressing has-miR-199b-5p (miR-199b-5p) were used to treat A549 or H299 cells. Cell motility was evaluated using wound scratch healing and transwell assays. Gene and protein expression were detected by quantitative real-time PCR (QRT-PCR) and Western blot. Target genes of miR-199b-5p were predicted through multiple-database analysis and validated.

Results

Transmission electron microscopy (TEM) demonstrated isolated exosomes had a typical bilayer membrane (30-100 nm). miR-199b-5p was highly expressed in lung cancer cells and detectable in the serum of lung cancer patients, with t

SUBMITTER: Liu B 

PROVIDER: S-EPMC12554135 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature