Deriving fibroblast cell lines from wing-punch biopsies of Australian eastern bent-winged bats (<i>Miniopterus orianae oceanensis</i>).

ABSTRACT: Cell cultures are a valuable tool for the study of in vitro disease processes, especially where such processes concern wild and/or threatened animal species. However, the collection of adequate samples for cell line preparation can be challenging under field conditions due to logistical challenges and access to equipment. In this paper, we describe the generation of fibroblast cell lines derived from wing-punch biopsies of Australian eastern bent-winged bats (Miniopterus orianae oceanensis), expanding on and modifying existing protocols. Twenty wing-punch biopsies were collected from free-ranging individuals in New South Wales in February 2024 and shipped to the University of Melbourne, Victoria, within 24 hours. To assess the impact of different preservation methods on sample integ

SUBMITTER: Langguth A 

PROVIDER: S-EPMC12554304 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature