Single-Cell Transcriptome Profiling Reveals Dynamic Cell Populations and Immune Infiltration in Moyamoya Disease.

ABSTRACT:

Background

The cellular composition and molecular mechanisms of the pathological arteries in Moyamoya disease (MMD) remain poorly understood. To improve our understanding of pathogenesis in MMD, we aimed to comprehensively map the cellular composition and molecular alterations within the pathological arteries of patients with MMD.

Methods

Superficial temporal artery samples were collected from patients with MMD (n=2) and healthy controls (n=3), yielding a total of 26 371 cells that were used for single-cell RNA sequencing. Differentially expressed genes, pathway enrichment, and cell-cell communication analyses were performed to examine disease-related changes. In vitro experiments were performed to validate the changes in endothelial cells (ECs) and smooth-muscle cells (SMCs

SUBMITTER: He S 

PROVIDER: S-EPMC12554407 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature