Establishment of chicken muscle and adipogenic cell cultures for cultivated meat production.

ABSTRACT:

Introduction

Cultured meat seeks to replicate the sensory and nutritional attributes l of conventional meat by developing structured muscle tissue using cell culture. This study focuses on the culture of chicken embryonic and muscle-derived mesenchymal stem cells (MSCs) to derive muscle, and fat, optimizing conditions for differentiation and integration.

Methods

We utilized monolayer and three-dimensional microcarrier-based cultures to produce muscle fibers and adipocytes while maintaining the extracellular matrix (ECM) integrity essential for tissue cohesion. Key pluripotency and myogenic markers (e.g., cOCT4, cMYOD, cMYH1E) were analyzed during differentiation, revealing dynamic gene expression patterns that underscore myogenesis.

Results

Myobla

SUBMITTER: Haach V 

PROVIDER: S-EPMC12554555 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature