Alpha-lipoic acid reduces oxidative damage and ameliorates follicular abnormalities in vitrified cat ovarian tissue.

ABSTRACT:

Introduction

Vitrification may induce oxidative damage, mitigating cryodamage is crucial for preserving follicular development and ovarian function. Alpha lipoic acid (α-ALA) is an antioxidant which exerts its antioxidant activity directly or indirectly.

Methods

In this study, we established a α-ALA concentration gradients (0, 100, and 150 μM) in the vitrification solution, assessed follicular abnormalities through hematoxylin and eosin (HE), analyzed cell apoptosis via TUNEL staining analysis, immunohistochemistry (IHC) and Masson staining, explored differential genes by transcriptome sequencing.

Results

Our findings revealed that α-ALA alleviated oxidative stress damage and decreased follicular abnormalities induced by vitrification, enhanced antioxidant capacity an

SUBMITTER: Yin S 

PROVIDER: S-EPMC12554562 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature