Targeted next-generation sequencing using bronchoalveolar lavage fluid samples for diagnosing pulmonary infections: a single-center retrospective study.

ABSTRACT:

Objectives

To evaluate the diagnostic value of targeted next-generation sequencing (tNGS) of bronchoalveolar lavage fluid (BALF) samples from patients with pulmonary infections (PIs).

Methods

This retrospective study included 110 patients diagnosed with suspected PIs, who underwent tNGS of BALF samples between February 2023 and January 2025. Conventional microbiological tests (CMTs), traditional culture, and tNGS were simultaneously performed, and the diagnostic efficiencies of the three PI methods were compared.

Results

A total of 110 BALF samples were obtained from 110 patients, including 80 with PIs and 30 without PIs. The detection sensitivities of tNGS, culture, and CMTs for the diagnosis of PIs significantly differed (P < 0.001). Further analysis showed t

SUBMITTER: Huang J 

PROVIDER: S-EPMC12554695 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature