Tear fluid proteomics: a comparative study of DIA and DDA mass spectrometry.

ABSTRACT:

Background

Mass spectrometry is a powerful technique for tear fluid proteomics, offering critical insights into its complex molecular composition. Traditional data-dependent acquisition (DDA) often favors high-abundance proteins because it selects only the most intense precursor ions within a given window during each scan cycle. A newer approach, data-independent acquisition (DIA), addresses this by fragmenting all precursor ions within defined mass windows, offering broader coverage and improved quantification. This study presents a systematic comparison of DDA and DIA workflows to assess their relative performance in detecting tear fluid proteins.

Methods

Tear fluid samples were collected from healthy individuals using Schirmer strips, processed using in-strip protein dige

SUBMITTER: Ahmed S 

PROVIDER: S-EPMC12554977 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature