Re-calibration of flow cytometry standards for plant genome size estimation.

ABSTRACT: Flow cytometry (FCM) and genome sequencing are complementary methods for estimating plant genome size (GS). However, discrepancies between the GS estimates derived from genome assemblies and FCM create ambiguity regarding the accuracy of these approaches. Approximately 12,000 plant GS measurements have been reported, with hardly any of them based on genome assemblies. Currently, FCM is the most frequently used method. Accurate GS estimation by FCM relies on internal standards with known GS values. However, previous GS calibrations, often based on incomplete reference genome assemblies, have led to significant discrepancies in GS estimates. Historically, the GS of a diploid plant species was estimated by doubling the size of a consensus genome assembly. However, consensus assemblies collaps

SUBMITTER: Soni A 

PROVIDER: S-EPMC12555019 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature