Induction of Tolerogenic Dendritic Cells by a Noncoding Oligonucleotide.

ABSTRACT: Tolerogenic dendritic cells (tolDCs) that dampen T cell responses can be induced from blood monocytes in vitro using factors such as Vitamin D3 (VitD), dexamethasone, IL-10, or rapamycin. However, challenges remain in obtaining robust and efficient generation of cell therapy-based tolDCs without compromising their viability. We recently reported that CCR2-dependent recruitment of monocytic cells, with the capacity to dampen T-helper responses, occurs in mice treated with a single-stranded oligonucleotide (ssON). Here, we investigated the effects of this immunomodulatory noncoding ssON on differentiating human monocytes towards DC in the presence of IL-4 and GM-CSF (moDC). The moDC differentiated in the presence of ssON upregulated CD1a but also increased their expression of PD-L1. The diff

SUBMITTER: Kamal K 

PROVIDER: S-EPMC12555023 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature