Effects of disaccharide and cationic lipid types on reverse transfection with lyophilized mRNA lipoplexes.

ABSTRACT: Recently, the development of messenger RNA (mRNA) therapeutics has received increased attention, and mRNA/cationic liposome complexes (mRNA lipoplexes) have been studied as effective mRNA delivery carriers. However, owing to the vast diversity of the mRNA and carrier components employed, establishing a comprehensive analysis of their efficacy remains a critical issue. Reverse transfection using lyophilized mRNA lipoplexes has the potential to simplify and automate the efficacy evaluation processes. The present study investigated the effects of cationic lipids in cationic liposomes and disaccharides as cryoprotectants during reverse transfection with lyophilized mRNA lipoplexes. Overall, five types of dialkyl or trialkyl cationic lipids were used to prepare cationic liposomes. The mRNA lipo

SUBMITTER: Shimizu R 

PROVIDER: S-EPMC12555113 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature