Alternative splicing immune signature for detecting HBV-related hepatocellular carcinoma in peripheral blood mononuclear cells.

ABSTRACT: Peripheral blood mononuclear cells (PBMCs) exhibit disease-specific alternative splicing changes that may serve as robust biomarkers for hepatocellular carcinoma (HCC). Nearly half of the 800,000 annual HCC cases arise from chronic HBV, which affects 254 million people globally, underscoring the urgent need for reliable, non-invasive diagnostic tools. We conducted a comprehensive meta-analysis of PBMC transcriptomes, identifying reproducible immune-related alternative splicing events (ASEs) altered in HBV-related HCC. We identified shifts in inferred immune cell composition and ASEs associated with HBV-related HCC and tumor stage. Using eight immune-related ASEs, we trained a random forest classifier on 120 samples from six independent studies to distinguish HBV-related HCC from chronic HB

SUBMITTER: Daniels NJ 

PROVIDER: S-EPMC12555792 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature