Chemoselective sulfonyl fluoride exchange (SuFEx)-induced macrocyclization of tyrosine-containing peptides in aqueous media.

ABSTRACT: Cyclic peptides generally exhibit enhanced metabolic stability, cell permeability, and binding affinity to biological targets compared to linear peptide sequences, making them attractive scaffolds for therapeutic development. Due to the chemical heterogeneity of peptides, the development of new, chemoselective methods for peptide macrocyclization remains a significant challenge. Here, we report a tyrosine-selective strategy for the synthesis of (STEMtides) in aqueous buffer under mild conditions. This method leverages sulfur fluoride exchange (SuFEx) chemistry to engage the phenolic side chain of tyrosine residues with sulfonyl fluorides, achieving efficient and chemoselective cyclization without the need for additional reagents. The approach is highly tolerant of native side chain functio

SUBMITTER: Seyrani H 

PROVIDER: S-EPMC12557407 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature