Invasin-functionalized PIC hydrogels enable long-term 3D culture of epithelial organoids.

ABSTRACT: Tissue stem cell (TSC)-derived epithelial organoids are typically cultured in Matrigel [T. Sato et al., Nature 459, 262-265 (2009)], an extracellular matrix-like hydrogel produced from Engelbreth-Holm-Swarm sarcoma cells. This tumor is grown in the mouse abdomen [R. W. Orkin et al., J. Exp. Med. 145, 204-220 (1977)]. Previously, we demonstrated that the Yersinia membrane protein Invasin, coated on transwells, replaces Matrigel by activating β1-integrins, allowing long-term expansion of primary epithelial cells as 2D organoid sheets [J. J. A. P. M. Wijnakker et al., Proc. Natl. Acad. Sci. U.S.A. 122, e2420595121 (2025)]. Here, we functionalize a synthetic polyisocyanide (PIC) hydrogel with the integrin-activating d

SUBMITTER: Wijnakker JJAPM 

PROVIDER: S-EPMC12557532 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature