A Metabolite Co-Delivery Strategy to Improve mRNA Lipid Nanoparticle Delivery.

ABSTRACT: Lipid nanoparticles (LNPs) effectively protect mRNA and facilitate its entry into target cells for protein synthesis. Despite these successes, cellular entry alone may not be enough for optimal protein expression, as mRNA translation also depends on the availability of essential metabolites, including metabolic energy sources, coenzymes, and amino acids. Without adequate metabolites, mRNA translation may be less efficient, potentially leading to higher dosing requirements or poorer therapeutic outcomes for LNP therapies. To address this, we develop a metabolite co-delivery strategy by encapsulating essential metabolites within mRNA LNPs, hypothesizing that our approach can uniformly improve mRNA delivery. Instead of adding a fifth component to the organic phase, our strategy involves mixin

SUBMITTER: Ma Y 

PROVIDER: S-EPMC12557834 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature