Giant grouper nervous necrosis virus subgenomic RNA3 is transcribed by a premature termination mechanism via intragenomic RNA-RNA pairing.

ABSTRACT:

Background

Nervous necrosis virus (NNV) is a bipartite positive-sense RNA virus that causes viral nervous necrosis disease in larval and juvenile marine fish. In its genome, the RNA1 segment encodes RNA-dependent RNA polymerase (RdRp) for viral RNA synthesis, while RNA2 encodes coat protein for viral particle assembly. In addition, an RNA1-derived subgenomic RNA3 region encodes the non-structural B2 protein, which antagonizes host RNA interference mechanisms. However, the mechanisms of RNA3 transcription remain unclear.

Methods

A DNA-based reverse genetic platform vector (pCMV-DRz) for recombinant (r) giant grouper (GG) NNV recovery was constructed. End sequences of GGNNV RNAs were determined by 5' rapid amplification of cDNA ends (RACE) and/or circular RNA sequencing approa

SUBMITTER: Cheng CA 

PROVIDER: S-EPMC12557850 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature