Rational design of promoter editing confers multipathogen resistance in rice.

ABSTRACT:

Background

Fine-tuned gene expression rather than alterations in the protein-coding region of a gene is responsible for the optimal performance conferred by many elite alleles in crops. Lesion mimic mutants (LMMs), a type of plant mutants with hyperactivated immune responses, often show enhanced resistance but with yield penalties. To fine-tune the expression level of LMM genes using promoter editing is of considerable interest in crop disease control.

Results

Here we demonstrate the power of predictive promoter editing in optimizing expression of the rice LMM gene RBL1, encoding a CDP-DAG synthase in phospholipid metabolism, by breaking immunity-growth trade-offs. Through bioinformatic analyses of open chromatin accessibility, we identify key cis-regulatory regions in the R

SUBMITTER: Han X 

PROVIDER: S-EPMC12557872 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature