Translation of nervous necrosis virus involves eIF4E but not RPS6 phosphorylation and viral particle assembly in remodeled microtubule-organizing center.

ABSTRACT:

Background

Nervous necrosis virus (NNV) is a bipartite positive-sense RNA virus that causes piscine viral nervous necrosis disease in larvae and juvenile marine fish. Although NNV has been studied for decades, the translational mechanisms underlying its propagation remain unclear.

Methods

The molecular mechanism of NNV translation was investigated using grouper brain (GB) cell line infected with giant grouper (GG) NNV strain. Bromouridine and puromycin labelling were used to detect newly synthesized viral RNAs and proteins in viral factories (VFs), respectively. In addition, immunocytochemical staining, Western blotting and RNA fluorescence in situ hybridization (FISH) following densitometry and statistical analyses were employed to determine the involvement of cellular comp

SUBMITTER: Bajpai V 

PROVIDER: S-EPMC12557899 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature