Correlative microscopy for in-depth analysis of calcium oxalate crystals in plant tissues.

ABSTRACT:

Background

Calcium oxalate (CaOx) crystals are commonly found in many plant species. These crystals vary in distribution and morphology and to elucidate their role in plants multiple methods have been applied. Raman imaging and polarized light microscopy (PLM) easily visualize the crystals within plant tissues, but both methods are limited in spatial resolution by the diffraction of light. To unravel the distinctive shape and morphology of CaOx crystals down to the nanoscale and how they are embedded within cells, high resolution scanning electron microscopy is needed. To grasp the full potential of multiple methods in CaOx studies, a novel and easy-to-build correlative sampling approach is presented on different nut species (pecan (Carya illinoinensis), Turkish hazel (Corylus colu

SUBMITTER: Niedermeier M 

PROVIDER: S-EPMC12557963 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature