Minimally invasive detection of early-stage opisthorchiasis-associated cholangiocarcinoma using label-free surface-enhanced Raman spectroscopy (SERS) of hamster serum.

ABSTRACT:

Background

Cholangiocarcinoma (CCA) is a deadly cancer often detected late. Current diagnostic methods, such as ultrasound and invasive biopsies, have limitations; there is a critical need for a rapid, minimally invasive and effective strategy for the early diagnosis and staging of CCA.

Methods

We aimed to address this need using serum samples and label-free surface-enhanced Raman spectroscopy (SERS) combined with machine learning. CCA development was induced in hamsters using a combination of Opisthorchis viverrini infection and administration of N-nitrosodimethylamine, with induction time courses spanning 1-5 month(s). Normal and pathological stages (inflammation, precancerous lesion, and CCA) were assigned based on histopathological features, as well as the expression of

SUBMITTER: Chaidee A 

PROVIDER: S-EPMC12558545 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature