Agglutinin chip screening of B cell surface biomarkers in Hashimoto's thyroiditis for therapeutic targeting.

ABSTRACT:

Background and objectives

Hashimoto's thyroiditis (HT) is a chronic autoimmune thyroid disorder characterized by B lymphocyte dysregulation and the production of autoantibodies. This study aimed to evaluate a targeted B cell depletion strategy by identifying and validating a disease-associated membrane glycoprotein selectively expressed on B cells.

Methods

B lymphocytes were isolated from peripheral blood samples of 32 individuals with HT and 40 age- and sex-matched healthy controls (HC). Membrane glycoprotein expression was profiled using a 38-lectin microarray, followed by differential analysis via liquid chromatography-tandem mass spectrometry. Tetraspanin-33 (TSPAN33) was identified for further investigation based on its expression pattern. Recombinant TSPAN33 protein wa

SUBMITTER: Ren JL 

PROVIDER: S-EPMC12558958 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature