Impact of Clostridium butyricum HADIG-CB003 dietary supplementation on the gut microbiota of Kunming mice.

ABSTRACT: Clostridium butyricum is a key anaerobic probiotic due to its ability to produce butyric acid and form spores. In this study, four strains were isolated from pig feces using tryptose sulfite cycloserine (TSC) plates and specific primers sequentially. The optimal strain, C. butyricum HADIG-CB003, identified via gas chromatography and enzyme tests, produced 1.54 g/L of butyric acid, with cellulase and amylase activities of 20.88 ± 1.27 U/mL and 16.04 ± 0.46 U/mL, respectively. In reinforced clostridial medium, it produced 2.48 L of gas per liter, with hydrogen making up 59.68% (1.48 L). The adhesion rate to porcine intestinal epithelial cells (IEC-J2) was 9.16% in vitro. Twelve 3-week-old male Kunming mice were split into two groups and fed a basal diet with or without 1 × 10¹⁰ CF

SUBMITTER: Pang Y 

PROVIDER: S-EPMC12559120 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature