Nardilysin in adipocyte regulates insulin sensitivity via HIF1α and PPARγ.

ABSTRACT: Obesity is a critical global health issue and a major risk factor for type 2 diabetes mellitus (T2DM). However, molecular mechanism by which obesity leads to T2DM has not been fully elucidated. Here, we investigated the role of adipocyte nardilysin (NRDC) in insulin sensitivity using adipocyte-specific NRDC-knockout mice (Adipo-KO). Despite no difference in body weight, Adipo-KO demonstrated enhanced glucose tolerance and insulin sensitivity exclusively under high fat diet (HFD) conditions. Epididymal white adipose tissue (eWAT) in Adipo-KO showed the decreased crown-like structures and F4/80-positive cells. Consistently, insulin-stimulated Akt phosphorylation was elevated in the eWAT of HFD-fed Adipo-KO and 3T3-L1 adipocytes with NRDC knockdown. In addition, NRDC knockdown led to a decrea

SUBMITTER: Matsuda S 

PROVIDER: S-EPMC12559285 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature