Actin cytoskeleton dynamics affect replication of human Metapneumovirus.

ABSTRACT: Human Metapneumovirus (hMPV) is a leading cause of viral respiratory infection with clinical and epidemiological relevance in pediatric, immunocompromised, and elderly populations. Little is known about hMPV in vitro replication processes and their relationship with cellular structures such as the cytoskeleton. Our goal was to evaluate the role of the actin cytoskeleton in hMPV replication at different stages of viral growth. hMPV was isolated in Vero cells from a clinical sample and identified as A₂ genotype. The cytopathic effect was detected by the appearance of cell rounding and refractory cell clusters. The growth curve showed that viral replication maximum level was between 48 and 72 hpi. The highest percentage of infected cells and intracellular hMPV-protein were detected

SUBMITTER: Rodriguez PE 

PROVIDER: S-EPMC12559302 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature