Ubiquitin-specific protease 7 regulates macrophage polarization via pyruvate kinase M2-mediated metabolic reprogramming in severe acute pancreatitis.

ABSTRACT: Severe acute pancreatitis (SAP) is a life-threatening inflammatory condition, with macrophage polarization playing a pivotal role in its pathogenesis. This study aims to investigate the role and underlying mechanism of ubiquitin-specific protease 7 (USP7) in regulating macrophage polarization in SAP. The expression of USP7 in the pancreas of SAP mice were assessed to determine its involvement in the disease. Histological evaluation, immunofluorescence, flow cytometry, and Western blotting were utilized to phenotype macrophages and assess the expression of inflammatory markers in both mouse models and cell cultures. Seahorse assays were employed to measure the extracellular acidification rates (ECAR) and oxygen consumption rates (OCR). The interaction between USP7 and pyruvate kinase M2 (PK

SUBMITTER: Wu Y 

PROVIDER: S-EPMC12559371 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature