Phenylamine-1,2-Dioxetanes: Promising Class of Chemiluminescent Luminophores for Aqueous Sensing and Bacterial Detection.

ABSTRACT: Chemiluminescence offers distinct advantages for bioimaging and sensing, notably by eliminating the need for external light excitation and minimizing background interference. While the original phenoxy-1,2-dioxetanes have served as the cornerstone of chemiluminescent probe design, their efficiency is significantly compromised in aqueous environments. In this study, we report the development and evaluation of phenylamine-substituted 1,2-dioxetanes as a new class of luminophores with markedly enhanced performance under physiological conditions. By incorporating amino-substituted benzoates, we achieved over 200-fold higher chemiluminescent emission compared to a conventional phenoxy analog. Among these, the mono-methylated probe NHMe-Diox exhibited improved properties, including fast chemiexc

SUBMITTER: David M 

PROVIDER: S-EPMC12559449 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature