LAMP using distance based paper device for quantitative detection of ESBL producing Escherichia coli in UTI patients.

ABSTRACT: Extended-spectrum beta-lactamases (ESBLs) producing E. coli are the most critical cause of antimicrobial resistance (AMR) and multidrug resistance (MDR) producing prolonged hospitalization and burden in treating nosocomial infection, in which the conventional culture regularly constitutes the gold standard diagnosis. In this study, we developed and validated a simple and inexpensive distance-based paper device (dPAD) LAMP assay for simultaneous screening and semi-quantifying the resistant bacterial load of ESBL-producing E. coli in 427 urine samples of patients with urinary tract infection (UTI). The device could measure the LAMP amplicons of bla_TEM gene and semi-quantify the degree of ESBL-producing E. coli in heavy (≥ 10² CFU/mL), light (10¹-10²

SUBMITTER: Saengsawang N 

PROVIDER: S-EPMC12559735 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature