CRL4^DCAF12 regulation of MCMBP ensures optimal licensing of DNA replication.

ABSTRACT: The minichromosome maintenance (MCM2-7) protein complexes are central drivers of genome duplication. Distinct protein pools, parental and nascent MCMs, and their precise equilibrium are essential to sustain error-free DNA replication. However, the mechanism responsible for generating these pools and maintaining their equilibrium remains largely unexplored. Here, we identified CRL4^DCAF12 as a factor controlling the assembly of nascent MCM complexes. During MCM biogenesis, MCMBP facilitates the assembly and transport of newly synthesized MCM3-7 subcomplexes into the nucleus. Once in the nucleus, the MCM2 subunit must be incorporated into the MCM3-7 subcomplex, while MCMBP needs to be removed. CRL4^DCAF12 facilitates the degradation of MCMBP and thereby regulates the asse

SUBMITTER: Yadav AK 

PROVIDER: S-EPMC12559739 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature