Cerebrospinal fluid extracellular vesicle-derived miR-9-3p in spinal cord injury with neuroprotective implications and biomarker development.

ABSTRACT: Spinal cord injury (SCI) often results in severe disability, and early detection of molecular changes is crucial for guiding treatment. In both rat and human samples, we observed a significant increase in cerebrospinal fluid (CSF)-derived extracellular vesicle (EV) miR-9-3p after SCI, prompting further investigation into its role. In a rat model, miR-9-3p levels were significantly lower at the injured spinal levels but higher in the motor cortex, where astrocytes showed the highest expression. Functional analyses revealed that miR-9-3p regulates energy metabolism, immune activity, and oxidative stress in neurons, inducing transcriptional changes suggestive of stress adaptation and synaptic remodeling. These findings demonstrate that EV-associated miR-9-3p modulates injury responses by redu

SUBMITTER: Tanaka T 

PROVIDER: S-EPMC12559747 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature