PRDX5 Regulates Mitochondrial Function and Nuclear Spreading in Myogenesis and Acts With PRDX3 to Delay Muscle Aging.

ABSTRACT:

Background

Skeletal muscle aging is associated with oxidative stress and mitochondrial dysfunction. Peroxiredoxins (PRDXs), particularly PRDX3 and PRDX5, are antioxidant enzymes that are uniquely localized to mitochondria. While PRDX3 has been reported to play a role in maintaining mitochondrial function in muscle, the specific function of PRDX5 in muscle remains unclear. This study investigated the role of PRDX5 in mitochondrial function, myonuclear distribution and muscle aging.

Methods

Myoblasts were isolated from wild-type (WT), Prdx3^-/-, Prdx5^-/- and Prdx3^-/-; Prdx5^-/- mice crossed with mitochondria reporter (mt-GFP) mice. Nuclear and mitochondrial positioning were evaluated using confocal and super-resolution lattice structur

SUBMITTER: Suh J 

PROVIDER: S-EPMC12559808 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature