Intersectin-1 enhances calcium-dependent replenishment of the readily releasable pool of synaptic vesicles during development.

ABSTRACT: Intersectin-1 (Itsn1) is a scaffold protein that plays a key role in coupling exocytosis and endocytosis of synaptic vesicles (SVs). However, it is unclear whether and how Itsn1 regulates these processes to support efficient neurotransmission during development. To address this, we examined the calyx of Held synapse in the auditory brainstem of wild-type and Itsn1 mutant mice before (immature) and after (mature) the onset of hearing. Itsn1 was present in the pre- and postsynaptic compartments at both developmental stages. Loss of function of Itsn1 did not alter presynaptic action potentials, Ca²⁺ entry via voltage-gated Ca²⁺ channels (VGCCs), transmitter release or short-term depression (STD) induced by depletion of SVs in the readily releasable pool (RRP) in either a

SUBMITTER: Yang YM 

PROVIDER: S-EPMC12559987 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature