Activation of TRPA1 and TRPM3 triggers Ca²⁺ waves in central terminals of sensory neurons and facilitates synaptic activity in the spinal dorsal horn.

ABSTRACT: Transient receptor potential ankyrin 1 (TRPA1) and melastatin 3 (TRPM3) are transduction channels of sensory neurons that play major roles in peripheral mechanisms of somatosensation, including thermosensation, chemosensation and nociception. Recent studies suggest that both channels also contribute to central mechanisms of pain processing at the spinal cord level. TRPA1 and TRPM3 are highly permeable for Ca²⁺, suggesting that they could regulate Ca²⁺ signalling at spinal synapses. However, information about TRPA1- and TRPM3-induced Ca²⁺ signalling in the dorsal horn (DH) of the spinal cord is lacking. Here, we describe a dual-colour technique for simultaneously measuring Ca²⁺ concentration ([Ca²⁺]_i) in central terminals of

SUBMITTER: Andrianov YE 

PROVIDER: S-EPMC12559988 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature