Comprehensive Mutational Landscape of Yeast Mutator Strains Reveals the Genetic Basis of Mutational Signatures in Cancer.

ABSTRACT: Spontaneous mutation rates and spectra are influenced by an intricate interplay of processes including DNA replication, proofreading, and diverse DNA damage repair pathways. Although significant progress has been made in characterizing the functions of individual genes involved in these processes, their direct effects on mutation rates and spectra remain unclear. In this study, we employed a systematic gene knockout approach coupled with mutation accumulation (MA) experiments and whole-genome sequencing (WGS) to investigate the mutational landscape of Saccharomyces cerevisiae. We targeted 136 gene-deletion strains encompassing nearly all known genes associated with DNA replication and repair. Analysis of 1,081 MA lines revealed that 114 of the 136 genes significantly influenced at least on

SUBMITTER: Liu L 

PROVIDER: S-EPMC12559997 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature