Native mass spectrometry of membrane proteins reconstituted in peptidiscs.

ABSTRACT: Membrane proteins and lipids are essential for a wide range of cellular processes, making their structural characterisation essential for understanding biological function. However, the amphipathic nature of membrane proteins poses a significant challenge for traditional structural biology techniques. Membrane mimetics offer an alternative approach to studying membrane proteins in more native-like environments. Among them, peptidiscs have emerged as a promising tool for stabilising membrane proteins, allowing reconstitution from detergent micelles into a detergent-free, native-like environment that preserves structural integrity. While peptidiscs have shown utility in techniques such as mass photometry and cryo-EM, their compatibility with native mass spectrometry (MS) remains largely unex

SUBMITTER: Deedwania A 

PROVIDER: S-EPMC12560048 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature