RAPPID-M: A Mix-and-Measure Bioluminescent Sandwich Immunoassay Based on Generic Antibody-Binding Protein M.

ABSTRACT: Homogeneous immunoassays that allow direct in-sample detection of biomarkers provide an attractive alternative to traditional heterogeneous immunoassays that require multiple washing and incubation steps. We previously reported the development of RAPPID, a bioluminescent sensor platform that uses split luciferase complementation to enable sandwich immunoassays directly in solution. Although RAPPID provides many benefits over traditional heterogeneous immunoassays, it requires protein G-mediated photoconjugation of split luciferase fragments to the Fc part of IgG monoclonal antibodies. Here, we report a new generation of RAPPID sensors (RAPPID-M) that do not rely on photoconjugation of protein G, but instead use a generic antibody-binding domain derived from protein M. The use of protein M

SUBMITTER: Swietlikowska A 

PROVIDER: S-EPMC12560131 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature