Rapid detection of herpes simplex virus types 1 and 2 using a G-quadruplex aptamer-based biosensor.

ABSTRACT: Herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), which cause oral and genital herpes in humans, are prevalent worldwide. ELISA, real-time PCR, and cytological assays are conventional methods for detecting these viruses, but they are expensive and time-consuming. The main purpose of this study was to design a specific G-quadruplex aptamer for the simple and rapid detection of HSV-1 and HSV-2. In this study, a specific aptamer was designed using bioinformatics tools for binding to the glycoprotein gD in HSV-1 and HSV-2. After evaluating the binding of the aptamer to gD, based on the stability scores of the secondary and tertiary structures and molecular docking, the aptamer AptNR88 was selected, and its binding to the target protein was confirmed experimentally using a colorimetric syst

SUBMITTER: Nouri S 

PROVIDER: S-EPMC12560281 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature