PADI2 exacerbates Mycoplasma ovipneumoniae-induced lung injury in sheep by suppressing M2 macrophage polarization.

ABSTRACT: Macrophages are considered the first line of defence against Mycoplasma pneumoniae infection in sheep, and their polarization significantly affects lung tissue damage and remodelling. Here, we collected sheep bronchoalveolar lavage fluid (BALF) 28 days after infection with Mycoplasma ovipneumoniae (MO). RNA-seq analysis revealed a significant increase in peptidyl arginine deiminase 2 (PADI2) expression in the MO group, and upregulated genes were significantly enriched in the "FoxO signalling pathway" and "AMPK signalling pathway". Immunohistofluorescence (IHF) staining revealed a significant increase in the number of macrophages, which were widely distributed in the alveolar cavity, in the MO infection group, and PADI2 was also positively expressed in the macrophages. In bone marrow-derive

SUBMITTER: Yan C 

PROVIDER: S-EPMC12560429 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature