In silico design of a multi-epitope pan vaccine targeting Schistosoma species.

ABSTRACT: Schistosomiasis remains a significant public health burden, necessitating the development of effective vaccines against it. In this study, a multi-epitope subunit vaccine was designed against adenylate kinase 2 protein and evaluated for its potential to elicit protective immunity against three Schistosoma species. CTL, HTL, and B-cell epitopes were identified using immunoinformatics tools and linked using AAY and KK linkers, respectively. The 50S ribosomal protein L7/L12, a known TLR4 agonist, was incorporated as an adjuvant to enhance immune activation in the vaccine. Molecular docking and molecular dynamics (MD) simulations demonstrated a strong binding affinity between the vaccine and human TLR4, supported by low RMSD and Rg values, indicating structural stability. The negative binding

SUBMITTER: Gandvi D 

PROVIDER: S-EPMC12560484 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature