Amino acid-assisted enzymatic hydrolysis of corn stover for microbial lipid production by Rhodotorula toruloides.

ABSTRACT:

Background

Efficient enzymatic hydrolysis of lignocellulosic biomass (LCB) is essential for maximizing the recovery of fermentable sugars for diverse biotechnological applications. However, pretreatment by-products including lignin interact with hydrolytic enzymes, blocking their access to substrates and leading to poor monomeric sugar recovery. This study evaluated the effects of all 20 exogenous amino acids (AAs) as additives to block lignin active sites and facilitate enzyme access to polysaccharide substrates for enhanced sugar recovery. The hydrolysates were subsequently tested for microbial lipid production by Rhodotorula toruloides CGMCC 2.1389 as a model application.

Results

The study found that most AAs enhanced enzymatic hydrolysis of 1% (w/v) H₂SO₄

SUBMITTER: Kamal R 

PROVIDER: S-EPMC12560600 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature