An Efficient Solid Phase Extraction Method for Purification and Analysis of Compound-Specific Plant Sugar Stable Hydrogen Isotope Values.

ABSTRACT:

Rationale

The stable hydrogen isotope composition (δ²H) of plant compounds can serve as environmental or metabolic proxies, but interpretations are hindered by insufficient mechanistic understanding. This can be improved by analyzing the δ²H values of metabolic intermediates, such as sucrose, which is the direct substrate of cellulose and a main transport sugar. However, current preparation methods for carbohydrates in general and sucrose in particular are not time-efficient.

Methods

We evaluated methods that use acetylation of soluble carbohydrate plant extracts to aid in purification as well as isotopic analysis of plant sugars such as sucrose. Extracts were obtained using either hot water or hot 80% ethanol. Acetylated extracts were then purified usi

SUBMITTER: Hugger S 

PROVIDER: S-EPMC12560618 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature