Mapping disease-specific vascular cell populations responsible for obliterative arterial remodelling during the development of pulmonary arterial hypertension.

ABSTRACT:

Aims

Pulmonary arterial hypertension (PAH) is a lethal pulmonary vascular disease characterized by arteriolar pruning and occlusive vascular remodelling leading to increased pulmonary vascular resistance and eventually right heart failure. While endothelial cell (EC) injury and apoptosis are known triggers for this disease, the mechanisms by which they lead to complex arterial remodelling remain obscure. We employed multiplexed single-cell RNA sequencing at multiple timepoints during the onset and progression of disease in a model of severe PAH to identify mechanisms involved in the development of occlusive arterial lesions.

Methods and results

Single-cell transcriptional analysis resolved 44 global lung cell populations, with widespread early transcriptomic changes at 1 wee

SUBMITTER: Cober ND 

PROVIDER: S-EPMC12560791 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature