Nucleolin perturbation alters membrane lipid homeostasis.

ABSTRACT: AS1411 is a G-rich DNA aptamer that targets the multifunctional RNA-binding protein nucleolin. AS1411 has both antiproliferative and cell size-regulating activities and has been evaluated for clinical utility, reaching phase II trials as an anticancer agent. The mechanisms underlying cell size effects of AS1411 are not well understood and broad characterization of its molecular effects is lacking. Here, we used a multi-omics approach to profile transcriptome, proteome and lipidome changes in AS1411-treated NIH-3T3 cells, which increase in size in response to the aptamer. We found that AS1411 caused downregulation of cholesterol biosynthesis pathway enzymes at both mRNA and protein levels, without an accompanying reduction in cellular cholesterol levels or cholesterol uptake. In addition, A

SUBMITTER: Zahavi EE 

PROVIDER: S-EPMC12560814 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature