Effects of a <i>Bacillus licheniformis</i> Fermentation Extract and Monensin on the Rumen and Hindgut Microbiota Composition of Lactating Dairy Cows.

ABSTRACT: This research reports ruminal and fecal microbiota composition of lactating dairy cows enrolled in a study aimed at investigating the effects of a fermentation extract derived from Bacillus licheniformis (BLFE), monensin (Rumensin^®; R), and their interactions on feed efficiency (FE, FE = milk yield/DMI). In a completely randomized design, 48 Holstein cows at 108 ± 35 days in milk were matched for parity and assigned to monensin (0 or 17.6 g/kg of DM) and BLFE (0 or 166 mg/kg of DM) in a 2 × 2 factorial arrangement. Treatments were fed daily for 63 d, including a 21 d adaptation period followed by a 42 d measurement period (P2). On d 38 and d 39 of P2, rumen-fluid (RF) and fecal samples were collected. DNA from RF and feces was sequenced using 16S rRNA gene-amplicon sequen

SUBMITTER: Hartoonian P 

PROVIDER: S-EPMC12560897 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature