Optimization of Loop-Mediated Isothermal Amplification for Avian Influenza Detection.

ABSTRACT: Avian influenza viruses (AIV) cause severe economic losses in poultry production and pose zoonotic threats, necessitating rapid, field-deployable diagnostics. While real-time PCR is the gold standard, its use is limited in resource-constrained settings. This study aimed to develop and validate optimized loop-mediated isothermal amplification (LAMP) protocols for AIV detection directly at sample collection sites. We optimized Real-Time RT-LAMP and colorimetric LAMP assays targeting the conserved M gene, using primers described in the literature. Analytical sensitivity was assessed with a plasmid standard (10⁶-10⁰ copies/μL); specificity was evaluated against 27 AIV strains (H1-H12) and heterologous avian viruses (Newcastle disease, infectious bronchitis, Gumboro, and l

SUBMITTER: Glazunova A 

PROVIDER: S-EPMC12560903 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature