Exosomal miRNAs Mediate Immune-Metabolic Interactions in the Hemocytes of the Pearl Oyster <i>Pinctada fucata martensii</i>.

ABSTRACT: Mollusks, such as bivalves, face increasing threats, such as disease, in aquaculture. Exosomes, widely derived from living cells carrying diverse bioactive molecules, affect the immune response. To overcome these challenges, bivalves utilize exosomal miRNAs as critical regulators of immune responses. This study investigates the role of exosomal miRNAs in modulating immune and metabolic responses in Pinctada fucata martensii following lipopolysaccharide (LPS) stimulation. Exosomes (75-150 nm) were isolated from hemolymph and characterized. High-throughput sequencing identified 30 differentially expressed miRNAs (DEMs) and 1349 differentially expressed genes (DEGs) in LPS-treated oysters, with significant enrichment in TNF, TLR/NF-κB, and metabolic pathways. This study revealed exosom

SUBMITTER: Wang P 

PROVIDER: S-EPMC12560909 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature