Assessing Extracellular Vesicle Turnover In Vivo Using Highly Sensitive Phosphatidylserine-Binding Reagents.

ABSTRACT: Extracellular vesicles (EVs) are emerging as crucial players in cell communication and hold great promise as biomarkers and therapeutic tools. However, their diversity makes it challenging to detect, classify, and utilize them effectively, which limits their clinical applicability. A key challenge is the lack of reliable markers to identify EVs consistently. In this study, a novel high-affinity phosphatidylserine (PS)-binding reagent is introduced for EV analysis. PS is known as a marker of apoptotic cells and activated platelets, but its presence on EVs is debated due to variations in lipid composition. By comparing multiple PS-binding reagents, including MFG-E8 derivatives and Annexin V, it is demonstrated that ≈90% of EVs in human and mouse blood carry PS. Using the optimized reagent, t

SUBMITTER: Flaskamp L 

PROVIDER: S-EPMC12561326 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature