EDNA- and eRNA-Based Detection of 2-Methylisoborneol-Producing Cyanobacteria and Intracellular Synthesis Dynamics in Freshwater Ecosystem.

ABSTRACT: Taste and odor (T&O) compounds in freshwater are frequently produced by certain cyanobacteria; however, their occurrence remains difficult to predict. This study examined the temporal and spatial variations in the mibC gene, which encodes a critical enzyme in the biosynthesis of 2-methylisoborneol (2-MIB), by analyzing environmental DNA (eDNA) and RNA (eRNA) in the North Han River, Republic of Korea, from July 2019 to October 2021. Surface water was sampled at twelve sites and analyzed for mibC DNA copy number, RNA expression, cyanobacterial cell density, and 2-MIB concentration using quantitative PCR (qPCR), microscopy, and gas chromatography-mass spectrometry (GC-MS). The mibC gene was present throughout the year, exhibiting peaks from late summer to early winter; hi

SUBMITTER: Kim K 

PROVIDER: S-EPMC12561784 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature