ICE<i>Cle</i>SHZ29: Novel Integrative and Conjugative Element (ICE)-Carrying Tigecycline Resistance Gene <i>tet</i>(X6) in <i>Chryseobacterium lecithinasegens</i>.

ABSTRACT: Background/Objectives: The global dissemination of tet(X) variants critically threatens tigecycline efficacy as a last-resort antibiotic. The aim of this study was to characterize a tet(X6)-carrying integrative and conjugative element (ICE) in a multidrug-resistant Chryseobacterium lecithinasegens strain, SHZ29, isolated from Shanghai, China. Methods: Minimum inhibitory concentrations (MICs) were determined by broth microdilution for SHZ29. Whole genomic sequencing and bioinformatic analysis were performed to depict the structure of the novel tet(X6)-carrying ICE. Inverse PCR and conjugation experiments were conducted to investigate the transfer ability of the ICE. Results: We depicted a novel tet(X6)-carrying ICE, named ICECle

SUBMITTER: Chen X 

PROVIDER: S-EPMC12561888 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature