Experimental Airway Allogenic Transplantation Model with Decellularized Cryopreserved Tracheas.

ABSTRACT: Background/Objectives: Tracheal replacement remains an unmet necessity in airway pathology treatment. We describe a decellularization and cryopreservation tracheal replacement protocol tested in vivo. Methods: A prospective experimental cohort study is conducted in which tracheas from white New Zealand rabbits (n = 32) are decellularized and cryopreserved, sterilized with gamma radiation, and tutorized with a stent. Bilateral, pedicled fasciomuscular flaps are harvested, implanting one cryopreserved and one non-cryopreserved in each of 16 rabbits, dividing them into four groups (remaining tracheas implanted at 2, 4, 8, and 12 weeks, respectively). After removal, the tracheas are both histologically and biomechanically evaluated. Results: Decellularization is effective,

SUBMITTER: Martinez-Hernandez NJ 

PROVIDER: S-EPMC12561892 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature